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KMID : 1025520040460060897
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Journal of Animal Science and Technology
2004 Volume.46 No. 6 p.897 ~ p.902
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Identification of Hanwoo Using 3`-tailed Primer Associated with Single Nucleotide Polymorphism(SNP) in Melanocortin 1 Receptor(MC1R) gene
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Kim Tae-Jung
Park S. D.
Lee Jae-Il
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Abstract
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To improve the methods used for the identification of Hanwoo, we performed a PCR using 3’-tailed primer associated with single nucleotide polymorphism(SNP) in Melanocortin 1 receptor(MC1R) gene. MC1R plays an important role in melanin synthesis and the SNP within MC1R was used as a target for PCR- RFLP studies previously.
A forward 3’-tailed primer, which matches with the template DNA of Hanwoo but not with others(black- haired; Holstein and Black angus) at the site of 594th base sequence, and one reverse primer were designed for this study. When use this primer set, a size of 343bp was amplified by PCR only in Hanwoo, not in Holstein and Black angus. This result suggests that the PCR using our 3’-tailed primer would be very accurate, easy, reproducible and economic method to discriminate between Hanwoo meat and other black-haired ones.
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KEYWORD
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MCIR gene, Coat color, Tailed-primer, Hanwoo
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